HEPATOTOXIC EFFECT OF 17-OXIMINO-5-ANDROSTEN-3β-YL BENZOATE ON RATS

  • Neelima Dhingra University Institute of Pharmaceutical Sciences, Panjab University
  • Vijayta D Chadha Centre for Nuclear Medicine, University Institute for Emerging Areas in Science and Technology, Panjab University
  • Anna Ara Khan University Institute of Pharmaceutical Sciences, Panjab University
  • Gousia Jan University Institute of Pharmaceutical Sciences, Panjab University

Abstract

Introduction and positioning of the hydroximino group on the parental steroid skeleton has significant effect on the activity profile of steroidal compounds. Earlier reports described the synthesis and evaluation of 17-Oximino-5-androsten-3ß-yl ester derivatives as potential cytotoxic agents on liver cancer cell lines (Hep-2) using Sulphorodamine B6 assay. The 17-Oximino-5-androsten-3?-yl benzoate displayed significant cytotoxicity against liver as compared to standard reference drugs. The current study was undertaken to investigate further the in vivo effect of the synthesized derivative on the liver of rats following one month treatment. Male SD rats were divided into two groups viz: normal control and 17-Oximino-5-androsten-3ß-yl ester derivative treated (dose 10mg/Kg body weight, intraperitoneally). After one month of treatment, the hepatotoxicity was assessed by estimating the levels of hepatic marker enzymes, oxidative injury and histopathological studies in the liver of control and treated rats. The study revealed a significant increase in the levels of glutamic oxaloacetic transaminase (GOT/AST), glutamic pyruvic transaminase (GPT/ALT) and alkaline phosphatase (ALP) in the serum and liver of 17-Oximino-5-androsten-3?-yl benzoate treated rats when compared to control animals. Further, increased lipid peroxidation (LPO) was accompanied by a decrease in glutathione (GSH) level. However, the activities of glutathione-s-transferase, (GST), superoxide dismutase (SOD) and catalase were found to increased significantly in the treated group. Histopathological observations also indicated marked alterations in the histoarchitecture of liver in response to 17-Oximino-5-androsten-3ß-yl ester derivative treatment. In conclusion, the synthesized compounds possess hepatotoxicity and exerts its cytotoxic action by oxidative cell injury.

Published
Apr 13, 2017
How to Cite
DHINGRA, Neelima et al. HEPATOTOXIC EFFECT OF 17-OXIMINO-5-ANDROSTEN-3β-YL BENZOATE ON RATS. International Journal of Drug Research and Technology, [S.l.], v. 5, n. 1, p. 2, apr. 2017. ISSN 2277-1506. Available at: <http://ijdrt.com/index.php/drug-research-and-technology/article/view/85>. Date accessed: 15 sep. 2019.
Section
Research Article
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