ANTITRYPANOSOMAL POTENCY OF METHANOL EXTRACT OF CASSIA AREREH DELILE ROOT BARK IN ALBINO RATS

  • Saidu Ibrahim Ngulde Department Of Veterinary Physiology, University Of Maiduguri, NIGERIA
  • Mohammed Bashir Tijjani Department Of Veterinary Physiology, University Of Maiduguri, NIGER
  • James Moses Ihopo Department Of Veterinary Microbiology And Parasitology, University Of Maiduguri, NIGERIA
  • Alhaji Mohammed Ya’uba Department Of Veterinary Microbiology And Parasitology, University Of Maiduguri, NIGERIA

Abstract

The methanol extract of the root bark of Cassia arereh Del. was investigated for antitrypanosomal activity in vitro and in vivo using Trypanosoma brucei brucei. In vitro antritrypanosomal potency was performed in triplicate in 96 wells microtitre plate, where the infected blood was treated with 1.25, 2.5, 5 and 10mg/ml concentration of plant extract and also with 3.5mg/ml diminazene aceturate and with dextrose saline. The oral lethal dose (LD50) was determined using Lorke’s method. Rats were inoculated with T. brucei brucei intraperitonealy and treated with the extract orally at 500 and 1000mg/ml (group A and B) while group C and D were given a single dose of 3.5mg/kg of diminazene aceturate and 0.5ml distilled water, respectively. In the in vitro study, there was significant extract concentration dependent activity against the parasite motility. The in vivo study revealed that treatment with the extract at 500 and 1000mg/ml did not affect the level of parasitaemia as swarming stage were maintained in the groups up to day 11 post treatment, but the diminazene treated group showed no parasitaemia after treatment. The packed cell volume of treated groups also decreased significantly. The oral LD50 was greater than 5000mg/Kg thus the extract has low toxicity and is relatively safe. It was concluded that the methanol extract of C. arereh Del. in vivo in rats has poor or no antitrypanosomal activity but has significant concentration dependent antitrypanosomal activity in vitro.


Keywords

Trypanosoma brucei, Cassia arereh, Methanol extract.

Published
Feb 28, 2017
How to Cite
NGULDE, Saidu Ibrahim et al. ANTITRYPANOSOMAL POTENCY OF METHANOL EXTRACT OF CASSIA AREREH DELILE ROOT BARK IN ALBINO RATS. International Journal of Drug Research and Technology, [S.l.], v. 3, n. 1, p. 6, feb. 2017. ISSN 2277-1506. Available at: <http://ijdrt.com/index.php/drug-research-and-technology/article/view/antitrypanosomal-potency-of-methanol-extract-of-cassia-arereh-delile-root-bark-in-albino-rats>. Date accessed: 15 sep. 2019.
Section
Research Article
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