ANTITRYPANOSOMAL POTENCY OF METHANOL EXTRACT OF CASSIA AREREH DELILE ROOT BARK IN ALBINO RATS
The methanol extract of the root bark of Cassia arereh Del. was investigated for antitrypanosomal activity in vitro and in vivo using Trypanosoma brucei brucei. In vitro antritrypanosomal potency was performed in triplicate in 96 wells microtitre plate, where the infected blood was treated with 1.25, 2.5, 5 and 10mg/ml concentration of plant extract and also with 3.5mg/ml diminazene aceturate and with dextrose saline. The oral lethal dose (LD50) was determined using Lorkeâ€™s method. Rats were inoculated with T. brucei brucei intraperitonealy and treated with the extract orally at 500 and 1000mg/ml (group A and B) while group C and D were given a single dose of 3.5mg/kg of diminazene aceturate and 0.5ml distilled water, respectively. In the in vitro study, there was significant extract concentration dependent activity against the parasite motility. The in vivo study revealed that treatment with the extract at 500 and 1000mg/ml did not affect the level of parasitaemia as swarming stage were maintained in the groups up to day 11 post treatment, but the diminazene treated group showed no parasitaemia after treatment. The packed cell volume of treated groups also decreased significantly. The oral LD50 was greater than 5000mg/Kg thus the extract has low toxicity and is relatively safe. It was concluded that the methanol extract of C. arereh Del. in vivo in rats has poor or no antitrypanosomal activity but has significant concentration dependent antitrypanosomal activity in vitro.
Trypanosoma brucei, Cassia arereh, Methanol extract.