International Journal of Drug Research and Technology

DEVELOPMENT AND VALIDATION OF HPTLC METHOD FOR THE ESTIMATION OF ANTIRETROVIRAL DRUGS AND THEIR PHARMACEUTICAL FORMULATIONS

Abstract

Naresh Chandra Joshi, Pradeep Kumar and Rakesh Kumar Jat

New, basic, delicate, exact & powerful superior dainty layer chromatographic (HPTLC) strategy was created for synchronous estimation of TENO & EMTRI in pharmaceutical measurement shapes. After improvement of plate, Camas TLC scanner III (examining speed 20 mm sec-1) was utilized for densitometry checking with WinCATs programming (opening miniaturized scale, 6 x 0.45 mm). Investigation of plate in absorbance mode at 274 nm was done for both medications all through trial. Framework was found to give smaller spots for TENO & EMTRI with RF (Retardation variable) estimation of 0.41±0.01and 0.56±0.01 separately. Data for adjustment plots demonstrated great direct association with r2 = 0.9985 & 0.9979 in fixation scope of 100-600ng/ml for both medications. Present technique was accepted by Conference on Harmonization (ICH) rules. Measurable investigation of information demonstrated that strategy is reproducible & exceptionally specific for estimation of TENO & EMTRI in quality control labs.